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Braz. j. microbiol ; 46(1): 113-116, 05/2015. tab, graf
Article in English | LILACS | ID: lil-748237

ABSTRACT

A modified colorimetric high-throughput screen based on pH changes combined with an amidase inhibitor capable of distinguishing between nitrilases and nitrile hydratases. This enzymatic screening is based on a binary response and is suitable for the first step of hierarchical screening projects.


Subject(s)
Aminohydrolases/analysis , Colorimetry/methods , High-Throughput Screening Assays/methods , Hydro-Lyases/analysis , Amidohydrolases/antagonists & inhibitors , Hydrogen-Ion Concentration
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